A cross-institutional collaboration has developed a technique to repartition carbon resources from carbohydrates to lipids in microalgae. It is hoped that this method can be applied to biofuel production. This discovery was the result of a collaboration between a research group at Kobe University’s Engineering Biology Research Center consisting of Project Assistant Professor KATO Yuichi and Professor HASUNUMA Tomohisa et al., and Senior Researcher SATOH Katsuya et al. at the Takasaki Advanced Radiation Research Institute of the Quantum Beam Science Research Directorate (National institutes for Quantum and Radiological Science and Technology).

These research results were published on April 9, 2021 in the international academic journal Communications Biology.

Main points

• Microalgae are highly capable of producing lipids by fixing atmospheric CO₂ via photosynthesis, making them promising candidates for biofuel production.
• In light/dark conditions (i.e. day and night), the majority of microalgae’s carbon resources obtained from CO₂ are accumulated as carbohydrates (starch). This makes it difficult to get microalgae to produce lipids.
• The researchers used ion beam mutagenesis to develop a strain of microalgae that can produce large amounts of lipids even under light/dark conditions.
• In this microalgae mutant, the starch debranching enzyme gene was disrupted, causing it to produce phytoglycogen, which is easily broken down. The carbon resources were then repartitioned from carbohydrate production to lipid production.

Research Background

Biofuels are renewable resources that have received much attention in the move towards creating more sustainable societies. Microalgae are photosynthetic organisms that are highly capable of producing lipids from carbon dioxide in the atmosphere, making them promising candidates for biofuel production. However, a Kobe University research group consisting of Project Assistant Professor Kato Yuichi and Professor Hasunuma Tomohisa et al. discovered that the majority of carbon resources were diverted to starch production instead of lipid production under light/dark conditions (i.e. day and night). This is a problem when cultivating microalgae species outside.

Research Methodology

For this research study, Project Assistant Professor Kato and Professor Hasunuma’s Kobe University research group collaborated with Senior Researcher Satoh et al. at the National institutes for Quantum and Radiological Science and Technology (QST). The researchers used the ion beam at QST’s Takasaki Advanced Radiation Research Institute to induce mutation in the microalgae. This enabled them to cultivate a new mutant strain called Chlamydomonas sp. KOR1 ^(*1), which can produce large quantities of lipids even in light/dark conditions.

The researchers discovered that this KOR1 strain has disruptions in the starch debranching enzyme ^(*2) gene ISA1, causing it to produce a different carbohydrate: phytoglycogen ^(*3) instead of starch (Figure 1).

Normally, microalgae synthesize and accumulate carbohydrates (starch) during light periods and break them down when it is dark. However, many carbohydrates accumulate that cannot be completely broken down. Contrary to this, the carbohydrate synthesized by KOR1 (phytoglycogen) was completely broken down during the dark period. The results of the KOR1 metabolome analysis ^(*4) revealed a total increase in intermediate metabolites in both the starch and lipid synthesis pathways (intermediate metabolites included fructose-6-phosphate, glucose-6-phosphate, acetyl-CoA and glycerol 3-phosphate). From this analysis, the researchers illuminated the metabolic mechanism underlying the increased lipid production that resulted from ISA1 gene disruption. In the KOR1 strain, the carbohydrate (phytoglycogen) was quickly broken down and intermediate metabolites subsequently induced the carbon resource to be repartitioned to lipid production (Figure 2).

Further Developments

In order to produce biofuels using microalgae, it is necessary to cultivate these organisms outside in the sunlight. However, there is an unavoidable decrease in lipid production under these light/dark conditions. The technique of ‘repartitioning carbon resources by disrupting the starch debranching enzyme gene’ developed through this research is one answer to this problem. It is hoped that this new method can contribute towards the large-scale implementation of biofuel production using microalgae.

Glossary

*1 Chlamydomonas sp. KOR1

Using the microalgae Chlamydomonas sp. JSC4 (isolated from brackish waters in Taiwan) as the parental strain, KOR1 is a mutant obtained via ion beam mutagenesis ^(*5). It can be cultivated in either freshwater or saltwater. With its ability to break down carbohydrates and convert them into lipids, it has both a high proliferation rate and a high lipid accumulation rate, demonstrating highly efficient lipid production.

*2 Starch debranching enzyme

Protein that slices through starch’s branch structure. It not only breaks down starch but also creates appropriate structures for synthesis.

*3 Phytoglycogen

Phytoglycogen has a highly branched structure compared to starch. It is also highly soluble in water, which means that it is easily broken down and is easy for intercellular enzymes to use.

*4 Metabolome analysis

A method of comprehensively analyzing ionic small molecules in a sample using CE-TOFMS (Capillary electrophoresis-time of flight mass spectrometry) apparatus.

*5 Ion beam mutagenesis

This involves hitting cells in a plant or microorganism with the accelerated ion molecules of various atoms (such as carbon) at tenths of the speed of light using a particle accelerator. This irradiation technique alters DNA, thus making it possible to create a breed with useful characteristics.

Acknowledgements

This research received financial support from the following:

• The Impulsing Paradigm Change through Disruptive Technologies (ImPACT) program (of the Cabinet Office of the Government of Japan).
• The Japan Science and Technology Agency (JST)’s Adaptable and Seamless Technology transfer Program (A-STEP).

Journal Information

Title

“Enhancing carbohydrate repartitioning into lipid and carotenoid by disruption of microalgae starch debranching enzyme”

DOI

10.1038/s42003-021-01976-8

Authors

Yuichi Kato, Tomoki Oyama, Kentaro Inokuma, Christopher J. Vavricka, Mami Matsuda, Ryota Hidese, Katsuya Satoh, Yutaka Oono, Jo-Shu Chang, Tomohisa Hasunuma*, and Akihiko Kondo
* Corresponding author

Journal

Communications Biology

• Access at the Kobe University Repository Kernel